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Hepatic Non-Parenchymal Cells


TRL provides human and animal hepatic non-parenchymal cells (NPCs) for research purposes. These cell types include, but are not limited to, sinusoidal endothelial cells (LSECs), Stellate cells, cholangiocytes, and Kupffer cells. NPCs play important roles in transport, metabolism, inflammatory response, disease modeling, and growth functions in the liver. The addition of NPCs to ADMETox studies may increase the physiological relevance of research.

Fresh Human NPCs

Fresh human liver non-parenchymal cells are provided in suspension. Each vial contains a mixture of liver non-parenchymal cells, which may include Kupffer cells, Stellate cells, LSECs, and cholangiocytes. The availability of fresh human NPCs depends on the availability of fresh human liver tissue. Register for our FreshAlert email list to receive a notification anytime we receive a fresh human liver tissue.

NPC Catalog Number Description
HUNPC Fresh Human NPCs (1mL)

Fresh Animal NPCs

Fresh animal liver non-parenchymal cells are sold in suspension.  Each vial contains a mixture of liver non-parenchymal cells, which may include Kupffer cells, Stellate cells, LSECs, and cholangiocytes.

Species and Strains Include:

  • Rat – Sprague-Dawley, Wistar Hannover, or Wistar
  • Mouse – CD-1 or C57Bl/6
  • Dog – Beagle
  • Monkey – Cynomolgus
NPC Catalog Number Description
RSNPC Fresh Rat NPCs (1mL)
MCNPC Fresh Mouse NPCs (1mL)
DBNPC Fresh Dog NPCs (1mL)
CYNPC Fresh Monkey NPCs (1mL)

Cryopreserved Human NPCs

Cryopreserved human liver non-parenchymal cells are provided in suspension.  Each vial contains a mixture of liver non-parenchymal cells, which may include Kupffer cells, Stellate cells, LSECs, and cholangiocytes. Check our current cryopreserved non-parenchymal cell inventory for availability.

NPC Catalog Number Description
HUCNP Cryopreserved human liver NPCs (1 million cells per vial)

Visit the Lonza e-store to place your order online or contact Customer Service at (800)638-8174.

Cryopreserved Human Hepatic Stellate Cells        

Human cryopreserved liver Stellate cells are characterized by FACS analysis, selecting for Stellate cell markers; specifically, vinculin, vimentin, and smooth muscle alpha actin.  Each vial contains a minimum of 100,000 viable and expandable cells.  Cells are usually sold at Passage 0 (P0) or P1, but no later than P3. Stellate cells consistently have a viability of 90% or better.  We also carry optimized growth medium specific to Stellate cells.

NPC Catalog Number Description
HUCLS Cryopreserved human Stellate cells (100,000 cells per vial)
MCST250 Cryopreserved human Stellate cell growth medium (250mL)

Visit the Lonza e-store to place your order online or contact Customer Service at (800)638-8174.

Stellate Cell Propagation Timeline

stellates pic

Cryopreserved Human Hepatic Kupffer Cells        

Human cryopreserved liver Kupffer cells are characterized by FACS analysis, selecting for macrophage cell markers; specifically, CD14, CD68, and CD11B.  Each vial contains a minimum of 1 million viable cells. Our Kupffer cells consistently have a viability of 90% or better.  We also carry optimized plating and maintenance medium specific to Kupffer cells.

 

NPC Catalog Number Description
HUCLK Cryopreserved human Kupffer cells (1 million cells per vial)
MCKP250 Cryopreserved human Kupffer cell plating medium (250mL)
MCKM250 Cryopreserved human Kupffer cell maintenance medium (250mL)

Visit the Lonza e-store to place your order online or contact Customer Service at (800)638-8174.

Kupffer Cell Propagation Timeline

kupffer pic

Resources

Stellate Cell Thawing and Plating Protocol

Kupffer Cell Thawing and Plating Protocol

Current Inventory

For more information about the use and application of NPCs contact us at (800)638-8174 or order.us@lonza.com.

We offer great discounts on bulk orders of cryopreserved hepatocytes. Contact us for more information.